In the name of ALLAH, the most beneficient, the most merciful

Agriculture Biotechnology (BT403)

Multiple Choice Questions (MCQs)

Objective Questions

  1. ________ refers to the state of being able to take up exogenous DNA from the environment.

    1. competence
    2. transformation
    3. microinjection
    4. Biolistics
  2. The lacZ gene codes for the ________ of the enzyme β-galactosidase.

    1. lacZ-α subunit
    2. lacZ-β subunit
    3. lacZ-∂ subunit
    4. lacZ-θ subunit
  3. The first transgenic mouse being created by injecting a gene for a art growth hormone into a mouse embryo in ________ year.

    1. 1988
    2. 1982
    3. 1998
    4. 1992
  4. The interaction of genes that are not alleles, in particular the suppression of the effect of one such gene by another is called ________.

    1. Epistasis
    2. Metastasis
    3. Codominance
    4. Multiallelic
  5. Which of the following is a tumor inducing Bacteria?

    1. Agrobacterium rhizogene
    2. Agrobacterium tumefaciens
    3. Agrobacterium subtilis
    4. E.coli
  6. Markers with visual identification are called?

    1. Single Nucleotide Polymorphism
    2. Molecular Markers
    3. Morphological Markers
    4. Plant Markers
  7. Commonly transformation occurs naturally in ________.

    1. bacteria
    2. viruses
    3. plants
    4. animals
  8. ________ is an example of genetic marker.

    1. GFP
    2. SNPs
    3. IAA
    4. FISH
  9. ________ is a gene or DNA sequence with a known location on a chromosome.

    1. Molecular marker
    2. DNA marker
    3. Protein marker
    4. Genetic marker
  10. The most preferred methods for plant transformation is ________?

    1. Agrobacterium mediated
    2. Heat shock method
    3. Electroporation
    4. Calcium chloride method
  11. ________ treatment in the presence of divalent cations such as Ca2+ (in CaCl2) prepares the cell membrane to become permeable to plasmid DNA ________?

    1. Centrifugation
    2. Heating
    3. Chilling
    4. All of the given
  12. ________ are natural vectors for genetic engineering?

    1. Gene gun
    2. Bacteria
    3. Viruses
    4. Phage
  13. Transduction is the process by which foreign DNA is introduced into a cell by a ________?

    1. Gene gun
    2. Virus
    3. Bacteria
    4. Phage
  14. Multiple forms of the same enzyme coded by the different genes is called ________.

    1. Isozyme
    2. Holoenzyme
    3. APoenzyme
    4. All of the given
  15. AFLP markers involves cleavage of DNA with ________ different enzymes.

    1. 1
    2. 2
    3. 3
    4. 4
  16. ________ and ________ are utilized by A, tumefaciens as sources of carbon and energy.

    1. opines, agropines
    2. auxin, cytokinin
    3. Ti plasmid, T-DNA
    4. amino acids, sugar derivatives
  17. ________ induces crown gall disease in Plants?

    1. A. tumefaciens
    2. A. rhizogenes
    3. E.coli
    4. All of the given
  18. The lacZ gene codes for the ________ of the enzyme β-galactosidase used in blue white screening.

    1. lacZ-θ subunit
    2. lacZ-β subunit
    3. lacZ-∂ subunit
    4. lacZ-α subunit
  19. Agrobacterium is a ________ Bacteria?

    1. Gram positive
    2. non-motile
    3. Non-ubiquitous
    4. All of the given
  20. Which of the following methods are used to make Bacterial cells competent to take the foreign plasmid DNA.________?

    1. Calcium chloride method
    2. Electroporation
    3. Heat shock method
    4. All of the given
  21. The genetic markers should be ________ and ________.

    1. polymorphic, reproducible
    2. rare, reproducible
    3. polymorphic, heterozygous
    4. good, resistable
  22. Which property belongs to morpological markers?

    1. Exhibit dominance
    2. Exhibit deleterious effects
    3. Exhibit pleiotropy
    4. All of the given
  23. Anything in the genome that is variable and can be used to compare individuals is called ________.

    1. Genetic marker
    2. Protein marker
    3. Molecular marker
    4. Morphological marker
  24. The explants of the plant must produce phenolic compounds for activation of virulence genes in ________ mediated transformation.

    1. agrobacterium
    2. virus
    3. bacteria
    4. yeast
  25. Bacteria having a plasmid containing ampicillin resistance gene can grow on a medium containing ________ antibiotic?

    1. cephalosporin
    2. Ampicillin
    3. kanamycin
    4. penicillin