Agriculture Biotechnology (BT403)

1. Which of the following methods are used to make Bacterial cells competent to take the foreign plasmid DNA.________?

   1. Calcium chloride method
   2. Electroporation
   3. Heat shock method
   4. All of the given

2. Transduction is the process by which foreign DNA is introduced into a cell by a ________?

   1. Gene gun
   2. Virus
   3. Bacteria
   4. Phage

3. The genetic markers should be ________ and ________.

   1. polymorphic, reproducible
   2. rare, reproducible
   3. polymorphic, heterozygous
   4. good, resistable

4. ________ is a gene or DNA sequence with a known location on a chromosome.

   1. Molecular marker
   2. DNA marker
   3. Protein marker
   4. Genetic marker

5. The lacZ gene codes for the ________ of the enzyme β-galactosidase used in blue white screening.

   1. lacZ-θ subunit
   2. lacZ-β subunit
   3. lacZ-∂ subunit
   4. lacZ-α subunit

6. ________ refers to the state of being able to take up exogenous DNA from the environment.

   1. competence
   2. transformation
   3. microinjection
   4. Biolistics

7. ________ and ________ are utilized by A, tumefaciens as sources of carbon and energy.

   1. opines, agropines
   2. auxin, cytokinin
   3. Ti plasmid, T-DNA
   4. amino acids, sugar derivatives

8. The most preferred methods for plant transformation is ________?

   1. Agrobacterium mediated
   2. Heat shock method
   3. Electroporation
   4. Calcium chloride method

9. Agrobacterium is a ________ Bacteria?

   1. Gram positive
   2. non-motile
   3. Non-ubiquitous
   4. All of the given

10. AFLP markers involves cleavage of DNA with ________ different enzymes.

    1. 1
    2. 2
    3. 3
    4. 4

11. Bacteria having a plasmid containing ampicillin resistance gene can grow on a medium containing ________ antibiotic?

    1. cephalosporin
    2. Ampicillin
    3. kanamycin
    4. penicillin

12. Which property belongs to morpological markers?

    1. Exhibit dominance
    2. Exhibit deleterious effects
    3. Exhibit pleiotropy
    4. All of the given

13. Anything in the genome that is variable and can be used to compare individuals is called ________.

    1. Genetic marker
    2. Protein marker
    3. Molecular marker
    4. Morphological marker

14. Markers with visual identification are called?

    1. Single Nucleotide Polymorphism
    2. Molecular Markers
    3. Morphological Markers
    4. Plant Markers

15. The interaction of genes that are not alleles, in particular the suppression of the effect of one such gene by another is called ________.

    1. Epistasis
    2. Metastasis
    3. Codominance
    4. Multiallelic

16. ________ are natural vectors for genetic engineering?

    1. Gene gun
    2. Bacteria
    3. Viruses
    4. Phage

17. Which of the following is a tumor inducing Bacteria?

    1. Agrobacterium rhizogene
    2. Agrobacterium tumefaciens
    3. Agrobacterium subtilis
    4. E.coli

18. Commonly transformation occurs naturally in ________.

    1. bacteria
    2. viruses
    3. plants
    4. animals

19. The lacZ gene codes for the ________ of the enzyme β-galactosidase.

    1. lacZ-α subunit
    2. lacZ-β subunit
    3. lacZ-∂ subunit
    4. lacZ-θ subunit

20. The first transgenic mouse being created by injecting a gene for a art growth hormone into a mouse embryo in ________ year.

    1. 1988
    2. 1982
    3. 1998
    4. 1992

21. The explants of the plant must produce phenolic compounds for activation of virulence genes in ________ mediated transformation.

    1. agrobacterium
    2. virus
    3. bacteria
    4. yeast

22. ________ is an example of genetic marker.

    1. GFP
    2. SNPs
    3. IAA
    4. FISH

23. ________ induces crown gall disease in Plants?

    1. A. tumefaciens
    2. A. rhizogenes
    3. E.coli
    4. All of the given

24. ________ treatment in the presence of divalent cations such as Ca2+ (in CaCl2) prepares the cell membrane to become permeable to plasmid DNA ________?

    1. Centrifugation
    2. Heating
    3. Chilling
    4. All of the given

25. Multiple forms of the same enzyme coded by the different genes is called ________.

    1. Isozyme
    2. Holoenzyme
    3. APoenzyme
    4. All of the given

Select a course code for Objective Questions:

ACC311 ACC501 BIF101 BIF401 BIF501 BIO101 BIO201 BIO202 BIO203 BIO204 BIO301 BIO302 BIO303 BIO401 BIO503 BNK603 BT101 BT201 BT301 BT302 BT401 BT402 BT403 BT404 BT405 BT406 BT501 BT504 BT505 BT604 CHE201 CHE301 CS001 CS101 CS201 CS202 CS204 CS301 CS302 CS304 CS311 CS401 CS402 CS403 CS408 CS501 CS502 CS504 CS506 CS508 CS601 CS602 CS604 CS605 CS607 CS609 CS610 CS614 CS707 CS710 ECO401 ECO402 ECO403 ECO404 EDU302 EDU401 EDU402 EDU501 EDU601 ENG101 ENG201 ENG301 ENG501 FIN611 FIN624 FIN625 ISL201 IT430 MCM101 MCM301 MGMT623 MGT101 MGT211 MGT301 MGT401 MGT402 MGT501 MGT502 MGT503 MGT510 MGT601 MGT603 MKT501 MKT610 MKT630 MTH101 MTH202 MTH302 MTH501 MTH601 MTH634 PAK301 PHY101 PHY301 PSY101 PSY403 PSY502 PSY512 SOC101 SOC301 SOC401 STA301 STA630 STA641 ZOO102 ZOO401 ZOO501 ZOO502 ZOO503 ZOO504 ZOO505 ZOO506 ZOO510 ZOO630

Select a course code for Subjective Questions:

ACC311 CS001 CS101 CS201 CS301 CS607 CS701 CS702 CS703 CS704 CS707 CS708 CS710 CS711 CS718 CS721 CS724 ECO401 ECO403 ENG101 ENG301 MGT703 MTH501 STA301