Methods in Molecular Biology (BIO203)

1. Why are vent ploymerase and Pfu more efficient than the Taq polymerase?

   1. Because of proofreading activity
   2. Because of more efficient polymerase activity
   3. Both of the given
   4. None of the given

2. Which of the following statements are true regarding PCR?

   1. Primer extension occurs at 72°C
   2. Denaturation involves heating at 90 to 98°C
   3. Annealing involves the binding of primer between 40 to 60°C
   4. All of the given

3. Suicide PCR is used in ________.

   1. Cytogenetics
   2. Human Genetics
   3. Paleogenetics
   4. None of the given

4. ________ enzymes have symmetrical recognition sequences but are of little value.

   1. Type III
   2. Exonucleases
   3. Type II
   4. Type I

5. Two pair of enzymes are used in ________ PCR.

   1. Nested
   2. Multiplex
   3. RT-PCR
   4. Conventional

6. The extension rate of Taq Polymerase enzyme is ________.

   1. 60 nt/sec
   2. 65 nt/sec
   3. 70 nt/sec
   4. 75 nt/sec

7. Melting of DNA template results in yielding ________ of DNA.

   1. Single strands
   2. Double strands
   3. Replicated strands
   4. All of the given

8. Assembly PCR is used to assemble two or more places of DNA into ________.

   1. One piece
   2. Two piece
   3. Three piece
   4. None of the given

9. Parental genetic diagnosis is not important for identification of ________.

   1. Chromosomal abnormality
   2. inherited mutation
   3. Familiar disorder
   4. Infectious diseases

10. ________ PCR is used for screening of bacterial or yeast clones for correct ligation or plasmid products.

    1. Asymmetric PCR
    2. Q-PCR
    3. Allele-specific
    4. Colony PCR

11. ________ PCR uses primers whose 3' ends encompass the SNP.

    1. Reverse Transcriptase PCR
    2. Assembly PCR
    3. Ligation mediated PCR
    4. Allele Specific PCR

12. Allele-specific PCR used for identifying of ________.

    1. SNPs
    2. Long genes
    3. Deletion Mutations
    4. Insertion Mutations

13. Real Time PCR is used to measure the quantity of a ________.

    1. RNA expression
    2. PCR product
    3. DNA amplification
    4. All of the given

14. A heat stable enzyme called Taq Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium ________.

    1. Escherichia coli
    2. Salmonella Typhi
    3. Thermus aquaticus
    4. Actinobacillus capsulatus

15. Genomic fingerprinting is a ________ medicine technique used to identify individuals by characteristics of their DNA.

    1. Alternative
    2. Toxicology
    3. Regenerative
    4. Forensic

16. Inverse PCR supports the amplification and identification of ________.

    1. Flanking sequences
    2. Genomic inserts
    3. Transposable elements
    4. All of the given

17. In ________ PCR there is very low probability of non-specific amplification of DNA.

    1. Nested
    2. Multiplex
    3. RT-PCR
    4. Conventional

18. Sequences of nucleotides which have a twofold axis of rotational symmetry is known as ________.

    1. binding site
    2. restriction site
    3. recognition site only
    4. Palindrome

19. Inverse PCR is used for the identification and amplification of ________.

    1. Long genes
    2. Flanking sequence
    3. Promote region
    4. Whole genome

20. ________ is used as a template to produce DNA in Reverse Transcripatase PCR.

    1. DNA
    2. RNA
    3. Protein
    4. All of the given

21. ________ refers to the study of ancient human, animal and plant remains.

    1. Molecular Biology
    2. Parasitology
    3. Pathology
    4. Paleontology

22. ________ tools have also allowed to perform prenatal genetic diagnosis.

    1. Molecular
    2. Microbiology
    3. Histology
    4. Radiology

23. What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?

    1. Precision and yield will be reduced
    2. Precision will be reduced, but yield will be increased
    3. Precision will be increased, but yield will be reduced
    4. Precision and yield will be increased

24. The process of reverse transcription reverse transcribes ________.

    1. RNA into DNA
    2. DNA into RNA
    3. DNA into DNA
    4. All of the given

25. Endonucleases are classified in to ________ main classes.

    1. 2
    2. 3
    3. 4
    4. 6

26. Degenerate Primers are used for the amplication of ________.

    1. Multiple genes
    2. Single gene
    3. Whole genome
    4. All of the given

27. If the primer sequence is TCG, what section of DNA will it anneal?

    1. AGC
    2. CAT
    3. GAC
    4. ATC

28. What happens during the Anneal step of PCR?

    1. Primers are created
    2. The primers attach to the target DNA region
    3. Primers copy the new DNA strand
    4. Primers sequence DNA

29. ________ PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated.

    1. Invers
    2. Allele-specific
    3. Colony
    4. Asymmetric

30. Denaturation is the process of ________.

    1. Heating between 72°C
    2. Heating between 40 to 60°C
    3. Heating between 90 to 98°C
    4. All of the given

31. In ________ is a polymerase chain reaction that actually takes place inside the cell on a slide

    1. Situ PCR (ISH)
    2. Nested PCR
    3. Colony
    4. RT-PCR

32. What happens in the Denature step of PCR?

    1. The DNA nucleotides are broken apart.
    2. The base-pairing rules for DNA are reversed.
    3. The double-stranded DNA is separated into two single strands of DNA.
    4. The DNA is returned to its natural setting.

33. Primers used for the process of polymerase chain reaction are ________.

    1. Single-stranded RNA oligonucleotide
    2. Single-stranded DNA oligonucleotide
    3. Double-stranded RNA oligonucleotide
    4. Double-stranded DNA oligonucleotide

34. ________ is used for the screening of bacterial of yeast clones for correct ligation or plasmid products.

    1. RT-PCR
    2. Real time PCR
    3. Nested PCR
    4. Colony PCR

35. At what temperature does annealing of DNA and primer take place?

    1. 54°C
    2. 96°C
    3. 42°C
    4. 74°C

36. ________ enzymes have symmetrical recognition sequencesbut are of little value.

    1. Type III
    2. Exonucleases
    3. Type II
    4. Type I

37. Mini Primer PCR uses a thermostable polymerase (S-Tbr) that can extend from short primers as short as ________ nucleotides.

    1. 14-15
    2. 5-6
    3. 7-8
    4. 9-10

38. The instrument used to run the PCR is known as ________.

    1. Centrifuge machine
    2. Incubator
    3. UV Transilluminator
    4. Thermocycler

39. What is the function of a primer?

    1. To identify the particular region of DNA to be copied by PCR.
    2. To copy DNA
    3. To create DNA nucleotides.
    4. To maintain the temperature of the PCR reaction.

40. Two sets of primers instead of one pair are uses in ________.

    1. Nested PCR
    2. Traditional PCR
    3. In-situ PCR
    4. None of the given

41. Methylation-specific PCR is used to identify patterns of DNA methylation at ________.

    1. CpG islands
    2. Promoter region
    3. AP Site
    4. Star site

42. The limitations of PCR are ________.

    1. Contamination risk
    2. Primer complexities
    3. Primer-binding site complexities
    4. All of the given

43. Enzyme that creates phosphodiester bond between 3' end of one DNA segment and the 5' another is known as ________.

    1. DNA polymerase
    2. DNA helicase
    3. DNA ligase
    4. RNA polymerase

44. Which one of the following is the source of pfu Polymerase?

    1. Thermococcus litoralis
    2. Thermococcus litoralis
    3. Pyrococcus furiosus
    4. T. aquaticus

45. Binding of primers to the single stranded DNA is called ________.

    1. Initialization
    2. Melting
    3. Binding
    4. Annealing

46. Molecular Detection methods can facilitate in detection of ________ in plants, animals and environment.

    1. trace element
    2. radioactive elements
    3. pigments
    4. pathogens

47. To amplify one strand of the original DNA more than the other, a special type of PCR is used which is known as ________.

    1. Asymmetric PCR
    2. Colony PCR
    3. In-situ PCR
    4. None of the given

48. For making cDNA ________ is used as a template.

    1. DNA
    2. RNA
    3. cDNA
    4. All of the given

49. ________ tools have also allowed to perform penatal genetic diagnosis.

    1. Molecular
    2. Microbiology
    3. Histology
    4. Radiology

50. Agarose extracted from sea weeds finds use in ________.

    1. Spectrophotometry
    2. Gel electrophoresis
    3. PCR
    4. Tissue culture

51. A recognition sequence usually ranges from ________ bases.

    1. 4-12
    2. 6-8
    3. 4-8
    4. 4-6

52. A technique for making millions of copies of a specific region of DNA.

    1. DNA ligase
    2. Restriction enzymes
    3. Gel electrophoresis
    4. Polymerase chain reaction

53. AFLP is a highly sensitive PCR-based method for detecting ________ in DNA.

    1. Polymorphisms
    2. Deletion
    3. Insertion
    4. Duplication

54. Non genomic material such as RNA can be detected by ________.

    1. In situ PCR
    2. Conventional PCR
    3. Western blotting
    4. Spectrophotometer

55. At what temperature does denaturation of DNA double helix takes place?

    1. 54°C
    2. 47°C
    3. 94°C
    4. 60°C

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