Multiple Choice Questions (MCQs)
________ is used as a template to produce DNA in Reverse Transcripatase PCR.
Denaturation is the process of ________.
Which one of the following is the source of pfu Polymerase?
The limitations of PCR are ________.
Enzyme that creates phosphodiester bond between 3' end of one DNA segment and the 5' another is known as ________.
Binding of primers to the single stranded DNA is called ________.
________ tools have also allowed to perform penatal genetic diagnosis.
________ enzymes have symmetrical recognition sequences but are of little value.
Which of the following statements are true regarding PCR?
Why are vent ploymerase and Pfu more efficient than the Taq polymerase?
A heat stable enzyme called Taq Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium ________.
Non genomic material such as RNA can be detected by ________.
In ________ is a polymerase chain reaction that actually takes place inside the cell on a slide
Molecular Detection methods can facilitate in detection of ________ in plants, animals and environment.
________ PCR uses primers whose 3' ends encompass the SNP.
The process of reverse transcription reverse transcribes ________.
Inverse PCR is used for the identification and amplification of ________.
The extension rate of Taq Polymerase enzyme is ________.
________ enzymes have symmetrical recognition sequencesbut are of little value.
Endonucleases are classified in to ________ main classes.
Two sets of primers instead of one pair are uses in ________.
________ PCR is used for screening of bacterial or yeast clones for correct ligation or plasmid products.
________ is used for the screening of bacterial of yeast clones for correct ligation or plasmid products.
Allele-specific PCR used for identifying of ________.
What happens in the Denature step of PCR?
Assembly PCR is used to assemble two or more places of DNA into ________.
At what temperature does annealing of DNA and primer take place?
What happens during the Anneal step of PCR?
Mini Primer PCR uses a thermostable polymerase (S-Tbr) that can extend from short primers as short as ________ nucleotides.
A recognition sequence usually ranges from ________ bases.
Degenerate Primers are used for the amplication of ________.
Genomic fingerprinting is a ________ medicine technique used to identify individuals by characteristics of their DNA.
Real Time PCR is used to measure the quantity of a ________.
At what temperature does denaturation of DNA double helix takes place?
________ refers to the study of ancient human, animal and plant remains.
________ PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated.
Parental genetic diagnosis is not important for identification of ________.
A technique for making millions of copies of a specific region of DNA.
Methylation-specific PCR is used to identify patterns of DNA methylation at ________.
________ tools have also allowed to perform prenatal genetic diagnosis.
Melting of DNA template results in yielding ________ of DNA.
In ________ PCR there is very low probability of non-specific amplification of DNA.
AFLP is a highly sensitive PCR-based method for detecting ________ in DNA.
The instrument used to run the PCR is known as ________.
Primers used for the process of polymerase chain reaction are ________.
Sequences of nucleotides which have a twofold axis of rotational symmetry is known as ________.
Inverse PCR supports the amplification and identification of ________.
What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
Two pair of enzymes are used in ________ PCR.
If the primer sequence is TCG, what section of DNA will it anneal?
What is the function of a primer?
For making cDNA ________ is used as a template.
Agarose extracted from sea weeds finds use in ________.
To amplify one strand of the original DNA more than the other, a special type of PCR is used which is known as ________.
Suicide PCR is used in ________.
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