In the name of ALLAH, the most beneficient, the most merciful

Introduction to Biotechnology (BT301)

Multiple Choice Questions (MCQs)

Objective Questions

  1. Which of the following statements are true regarding Fluorescence in situ hybridization (FISH)?

    1. It is a cytogenetic technique
    2. It uses fluorescent probes
    3. Fluorescent probes bind directly to the chromosomes
    4. All of the given
  2. Synthesis of cDNA from RNA is done using ________.

    1. RT-PCR
    2. inverse-PCR
    3. reverse-PCR
    4. qPCR
  3. cDNA library is representative of the ________ population of the derived host

    1. RNA
    2. DNA
    3. DNA & RNA
    4. proteins
  4. In order to differentiate the rare mutations from SNPs (single nucleotide polymorphism) the allele frequencies should be?

    1. 0.5%
    2. 1% or high
    3. 0.1%
    4. 0%
  5. cDNA is sequence which lacks ________ sequences.

    1. introns
    2. exons
    3. untraslated exons
    4. exons only
  6. Which of the following technique is used for Haploid genome mapping?

    1. RH Mapping
    2. Happy Mapping
    3. STS Mapping
    4. AFLP Mapping
  7. The principle of Chain termination method relies on ________.

    1. Use of chemicals for base specific cleavage
    2. Use of dNTPs for chain termination
    3. Use of ddNTPs for chain termination
    4. Use of Phosphorous-32 for chain termination
  8. The loss of plasmids due to defective partitioning is called

    1. Segregative stability
    2. Segregative instability
    3. Non-segregative instability
    4. Non-segregative stability
  9. In which of the following techniques Fluorescence microscopy is used to find out the fluorescent probe bound to the chromosomes?

    1. ALFP Mapping
    2. RFLP Mapping
    3. FISH (Flourescent insitu Hybradization)
    4. Happy Mapping
  10. Which of the following is used as a Landmark for physical map integration of genome?

    1. Genetic Markers
    2. Morphological Markers
    3. Number of Nucleotides
    4. All of the given
  11. Any Fragment in the genome can be used for STS mapping if it is ________?

    1. Unique sequence
    2. Polymorphic sequence
    3. Homologous sequence
    4. Universal Sequence
  12. In South-western and north-western blotting screening is carried out without using an antibody, by incubating the membranes with

    1. Radiolabelled double stranded DNA probe
    2. Radiolabelled double stranded RNA probe
    3. Radiolabelled single stranded DNA probe
    4. Unlabelled double stranded DNA probe
  13. The rate of synthesis of a particular protein will depend on the

    1. Mobile-state of mRNA
    2. Steady-state of DNA
    3. Steady-state of rRNA
    4. Steady-state of mRNA
  14. What should be the GC content of the primers used for RAPDs (Random amplified polymorphic DNA)?

    1. 50-80%
    2. 20%
    3. 30-40%
    4. 0-10%
  15. RFLP mapping is based on restriction-site ________ between ________ chromosomes?

    1. Variation, homologous
    2. No Variations, homologous
    3. Variation, Non-homologous
    4. No Variations, Non-homologous
  16. If the amino acid sequence changes from Cysteine to ________, it can increase the stability of the expressed protein.

    1. Glycine
    2. Serine
    3. Valine
    4. Lysine
  17. Maps that provide specified detail about the number of bases and distance that exists between genetic markers are ________?

    1. Physical Maps
    2. Genetic Maps
    3. Linkage Maps
    4. All of the given
  18. For ________ libraries, the correct orientation can be achieved by self-priming method by adding linker molecule to double stranded cDNA

    1. protein expression
    2. mRNA expression
    3. genome expression
    4. cDNA expression
  19. RH mapping can be used to identify ________?

    1. Locus of a gene
    2. Distances between different sites within a gene
    3. Distances between genes
    4. All of the given
  20. Physical Mapping was devised to check ________ between physical and linkage mapping?

    1. Similarity
    2. Correlation
    3. Dissimilarity
    4. All of the given
  21. ________ was the first mapping technique used in physical mapping?

    1. RFLP mapping
    2. STS mapping
    3. Happy mapping
    4. RH mapping
  22. What should be the approximate length of the primers used for RAPDs (Random amplified polymorphic DNA?

    1. 10 bps
    2. 18-20 bps
    3. 22-30 bps
    4. >30 bps
  23. ________ depends upon transcription of appropriate gene, efficient translation of mRNA and in many cases, post-translational processing and compartmentalization of nascent polypeptide.

    1. Synthesis of genome
    2. Synthesis of functional DNA
    3. Synthesis of functional RNA
    4. Synthesis of functional protein
  24. For the detection of single base nucleotide substitution, whic of the following mapping should be used?

    1. SNP mapping
    2. RFLP mapping
    3. RH mapping
    4. Happy mapping
  25. In chromosome jumping, the DNA of interest is identified, cut into fragments with restriction enzymes and

    1. Straighten
    2. Breaks down
    3. Elongates
    4. Circularized
  26. If DNA library is established using expressionvectors, each individual clone can be expressed to yield a

    1. Dipeptide
    2. Monopeptide
    3. Polypeptide
    4. Nucleotide
  27. Functional complementation is also possible in

    1. Artificial animals and plants
    2. Non-Transgenic animals and plants
    3. Transgenic animals and plants
    4. Transgenic mitochondria
  28. The methylation of Guanine at N-7 position in Maxam-Gilbert method is done by using ________.

    1. Formic acid
    2. Hydrazine
    3. Dimethyl sulphate
    4. Piperidine
  29. Which of the following is called as dideoxynucleotide chain termination method?

    1. Sanger's method
    2. Maxam-Gilbert method
    3. Edman's method
    4. Automated sequencing method
  30. Which of the following technique resembles with Amplified fragment length polymorphisms (AFLP) technique that detects genomic restriction fragments?

    1. RFLP (Restriction fragment lebgth polymorphism) technique
    2. SNPs (single nucleotide polymorphism) technique
    3. PCR (polymerase chain reaction) technique
    4. STS (sequenced tag sites) technique
  31. Major screening strategies involve ________.

    1. Genetic methods
    2. Sequence-dependent screenings
    3. Screening expression libraries
    4. All of the given
  32. In Sequenced tag site (STS) mapping, which of the following is not used?

    1. Southern blotting
    2. Restriction endonucleases
    3. Probes
    4. All of the given
  33. Chromosome Walking, In human genome, the starting point may be

    1. Cleaved Amplified Polymorphic Sequence (CAPS)
    2. single-nucleotide polymorphisms (SNPs)
    3. restriction fragment length polymorphism (RFLP)
    4. Amplified fragment length polymorphism (AFLP)
  34. Genomic DNA library is constructed using ________ cloning vectors.

    1. low capacity
    2. high capacity
    3. low copy number
    4. low copy and capacity
  35. In Chain termination method, we rely on ________ capabilities of DNA polymerase enzyme.

    1. Two
    2. Three
    3. Four
    4. Five
  36. To isolate specific clone, PCR is carried out with gene-specific primers

    1. gene-specific primers
    2. simple primers
    3. special primers
    4. universal primers
  37. Which of the following Mapping technique is based on the analysis of approximately Haploids samples using the Polymerase chain reaction?

    1. RH Mapping
    2. Genetic linkage mapping
    3. STS Mapping
    4. Happy Mapping
  38. Immunochemical screening involves the use of

    1. PCR
    2. Antigens
    3. Antibodies
    4. DNA
  39. A great advantage of hybridization for library screening is that it is

    1. Difficult
    2. Versatile
    3. Good
    4. Unique
  40. Amplified fragment length polymorphisms (AFLP) detects genomic restriction fragments and in that respect resembles to ________ technique?

    1. Happy Mapping
    2. STS mapping
    3. SNPs Mapping
    4. RFLP mapping
  41. Among the following, ________ act as chain terminator in the Chain termination method.

    1. Dideoxynucleotides
    2. DNA
    3. Deoxynucleotides
    4. Exogenous
  42. Which of the following is a cytogenic technique of genome mapping?

    1. ALFP Mapping
    2. RFLP Mapping
    3. FISH (Flourescent insitu hybradization)
    4. Happy Mapping
  43. Chain termination method was developed by Sanger et al. in ________.

    1. 1975
    2. 1976
    3. 1977
    4. 1978
  44. The high expression of insulin leads to the accumulation of aggregation of target proteins known as ________.

    1. Immunoglobulins
    2. Hormones
    3. Inclusion bodies
    4. Proteases