Multiple Choice Questions (MCQs)
________ enzymes have symmetrical recognition sequences but are of little value.
________ refers to the study of ancient human, animal and plant remains.
In ________ PCR there is very low probability of non-specific amplification of DNA.
________ PCR is used for screening of bacterial or yeast clones for correct ligation or plasmid products.
Mini Primer PCR uses a thermostable polymerase (S-Tbr) that can extend from short primers as short as ________ nucleotides.
Genomic fingerprinting is a ________ medicine technique used to identify individuals by characteristics of their DNA.
The instrument used to run the PCR is known as ________.
At what temperature does denaturation of DNA double helix takes place?
________ PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated.
Inverse PCR supports the amplification and identification of ________.
To amplify one strand of the original DNA more than the other, a special type of PCR is used which is known as ________.
For making cDNA ________ is used as a template.
Suicide PCR is used in ________.
A technique for making millions of copies of a specific region of DNA.
Two sets of primers instead of one pair are uses in ________.
Which of the following statements are true regarding PCR?
________ is used for the screening of bacterial of yeast clones for correct ligation or plasmid products.
Methylation-specific PCR is used to identify patterns of DNA methylation at ________.
What happens during the Anneal step of PCR?
Primers used for the process of polymerase chain reaction are ________.
Assembly PCR is used to assemble two or more places of DNA into ________.
Non genomic material such as RNA can be detected by ________.
Molecular Detection methods can facilitate in detection of ________ in plants, animals and environment.
Why are vent ploymerase and Pfu more efficient than the Taq polymerase?
Sequences of nucleotides which have a twofold axis of rotational symmetry is known as ________.
A heat stable enzyme called Taq Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium ________.
If the primer sequence is TCG, what section of DNA will it anneal?
What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
________ is used as a template to produce DNA in Reverse Transcripatase PCR.
Real Time PCR is used to measure the quantity of a ________.
Enzyme that creates phosphodiester bond between 3' end of one DNA segment and the 5' another is known as ________.
The extension rate of Taq Polymerase enzyme is ________.
Binding of primers to the single stranded DNA is called ________.
Allele-specific PCR used for identifying of ________.
________ enzymes have symmetrical recognition sequencesbut are of little value.
Two pair of enzymes are used in ________ PCR.
________ tools have also allowed to perform penatal genetic diagnosis.
Agarose extracted from sea weeds finds use in ________.
What is the function of a primer?
AFLP is a highly sensitive PCR-based method for detecting ________ in DNA.
The limitations of PCR are ________.
At what temperature does annealing of DNA and primer take place?
Degenerate Primers are used for the amplication of ________.
________ PCR uses primers whose 3' ends encompass the SNP.
A recognition sequence usually ranges from ________ bases.
The process of reverse transcription reverse transcribes ________.
Denaturation is the process of ________.
What happens in the Denature step of PCR?
Which one of the following is the source of pfu Polymerase?
Endonucleases are classified in to ________ main classes.
________ tools have also allowed to perform prenatal genetic diagnosis.
Melting of DNA template results in yielding ________ of DNA.
Parental genetic diagnosis is not important for identification of ________.
Inverse PCR is used for the identification and amplification of ________.
In ________ is a polymerase chain reaction that actually takes place inside the cell on a slide
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